FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6276722
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC121250.19317408035595648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG63950.067.930526
GTATAGG154900.067.470376
CTATAGG97050.067.323276
CGTATAA85950.066.861415
CGTATAG94450.066.621295
TTATAGG142050.066.1868746
TATAGGG140950.066.181247
GGTATAC83200.066.002475
GCTATAG94500.065.9938355
GCTATAC80000.065.975565
GGTATAG130600.065.722685
CTTATAG88150.065.708555
CCTATAC58000.065.551925
TTATAGC93850.065.481916
CTATAAC71750.065.420596
GGTATAA107850.065.284115
CTATACC69450.065.2704546
CGTATAC75550.065.1393665
TTTATAG119400.064.886345
CATATAG110300.064.880475
Produced by FastQC (version 0.11.5)