FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079624
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC64370.1577841487352756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG41400.066.665736
CGTATAG57450.066.3685765
CTTATAG54650.065.864475
TTATAGG93450.065.8060156
CTATAGG63350.065.709236
CGTATAA55650.065.686655
GGTATAC53350.065.502385
GTATAGG102400.065.144356
CTATAAC43850.064.855526
CTTATAC50650.064.850335
GCTATAC46150.064.806775
GGTATAG91850.064.6672745
TTATAGC60200.064.382716
TATAGGG94400.064.252077
CTATACC41400.064.130916
TTATACG55800.064.068226
TATAGGC76150.064.032877
CGTATAC44100.063.853295
GGTATAA73100.063.7401165
CCTATAC36400.063.7144785
Produced by FastQC (version 0.11.5)