FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555161
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC50420.14182198780870964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG83200.066.3801046
CTATACG35350.066.193576
GGTATAG80000.065.66885
TATAGGG79650.065.517317
CGTATAG52200.065.4640965
GCTATAC40450.065.370595
CTTATAG47800.065.3435445
CGTATAA51650.065.2131355
GTATAGG89800.065.162656
GGTATAC43000.064.910295
CTATACC35050.064.8641056
CTATAGG52750.064.781536
CTATAAC38250.064.649796
GGTATAA59850.064.2847065
CGTATAC38700.064.259575
TTATAGC54800.064.081446
TATAGGC62100.063.982287
TATACGG58650.063.8100057
CCTATAC30950.063.737875
CTTATAC47100.063.715565
Produced by FastQC (version 0.11.5)