FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5627359
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG132150.065.6700066
GGTATAG127150.065.666455
CGTATAG78550.065.3268055
GTATAGG144900.065.2506946
CTTATAG76600.064.84365
TATAGGG128700.064.628697
TTATAGC87200.064.422356
CTATAGG78600.064.261696
CTATACG56200.063.858486
CTATAAC51200.063.7410476
GTTATAG134050.063.4345135
GGTATAA98700.063.153595
GCTATAC64000.063.126185
GTATAGC90800.062.7156686
CGTATAC61150.062.6361665
CGTATAA79100.062.3519635
TATAGCG92350.062.1532367
CTATAGC61650.061.8444756
CTTATAC69400.061.843295
GGTATAC77300.061.812845
Produced by FastQC (version 0.11.5)