FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420306
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC79000.2309734859980364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC51200.066.539316
CTATACG37250.066.38696
TTATAGG84300.066.386786
CTTATAG46400.066.336715
GGTATAG71800.066.155295
CTATAGG56950.065.901496
TATAGGG81750.065.889447
CGTATAG50650.065.88075
GTATAGG91950.065.7326666
CGTATAA46750.065.690445
GCTATAC42300.065.489865
GGTATAC45550.065.424515
GGTATAA59050.065.334845
CTATAAC38250.064.742746
CGTATAC38800.064.546185
CTTATAC44950.064.430265
GCTATAG54700.064.327975
CTATAGC38350.064.300296
CCTATAA34850.064.2342155
TATAGGC66900.064.202977
Produced by FastQC (version 0.11.5)