FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4726678
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC121860.2578132041150254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG49800.067.355986
CGTATAG70900.066.40275
GGTATAG102550.066.236985
CTATAGG76750.066.126566
CTTATAG64000.066.07445
TTATAGC65600.066.062416
GTATAGG117600.065.9394456
CTATAAC50750.065.681616
TTATAGG114100.065.571046
CGTATAC55250.065.396575
GGTATAC61000.065.367515
GCTATAC58850.065.140495
TATAGGG114800.065.0791247
GGTATAA83400.065.047815
CGTATAA67400.065.024665
TATAACG67600.064.6246267
TATAGGC90100.064.557967
CCTATAC42000.064.541545
GCTATAG72950.064.4407965
GTATAGC69600.064.426676
Produced by FastQC (version 0.11.5)