FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6597-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724907
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC114550.20009058662437662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG79100.066.82115
CGTATAG90550.066.561395
GGTATAG130550.066.209675
CTATACG61100.066.12516
GTATAGG153100.065.916826
GGTATAC77800.065.8693855
TTATAGC87700.065.812736
TTATAGG140500.065.8031166
CTATAGG94050.065.497716
TATAGGG142000.065.154437
GCTATAC65200.064.864085
CGTATAA77300.064.802115
GCTATAG95750.064.663635
CGTATAC59150.064.638465
CTATAAC57050.064.3812266
GTATAGA109150.064.256386
CTATAAG67650.064.2213446
GGTATAA98500.063.959215
TATAGGC115000.063.5095867
TATAGTC111750.063.415957
Produced by FastQC (version 0.11.5)