FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6638986
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC102910.155008611254791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG66550.066.640476
CTATAGG96450.066.615276
GTATAGG168150.066.540226
TTATAGG148350.066.4385766
CGTATAA86800.066.244375
CTATAAC64400.066.204086
CTTATAG89600.066.165045
CCTATAC60450.065.843495
GGTATAG151950.065.693165
CTATACC65400.065.512666
CGTATAG98100.065.2809145
GGTATAA118650.065.264465
TTATAGC97300.065.0987246
TATAGGG156550.064.990677
GCTATAC75350.064.706275
GTATAGA108350.064.431546
AGTATAG128600.064.348835
GGTATAC88750.064.079495
CGTATAC75450.064.064255
GCTATAG105800.064.000975
Produced by FastQC (version 0.11.5)