FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6439285
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG60500.067.409796
CTATAGG88600.066.6374746
GTATAGG139700.066.5985956
CTTATAG80800.066.317365
TTATAGG137500.066.2142266
CGTATAG87350.066.109525
CGTATAA87950.065.976665
TTATAGC97550.065.901926
CTATACC62450.065.808996
CCTATAC52800.065.6477055
GCTATAC75200.065.6279755
CGTATAC69950.065.303325
TATAGGG130050.065.058697
GGTATAC80500.064.913425
CTTATAC82400.064.8174065
TATAGGC113300.064.7672357
ATTATAG118450.064.75655
GGTATAG131100.064.751065
GGTATAA107950.064.607415
TTATAGT123450.064.202016
Produced by FastQC (version 0.11.5)