FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6299963
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA87100.067.027965
CTATACG63400.066.9798056
GTATAGG134850.066.612926
GCTATAC77950.066.504335
TTATAGG129950.066.056066
CCTATAC62600.066.04825
CTATAGG87150.066.025696
CGTATAG85150.065.851675
GGTATAA103900.065.7513355
CTATACC67750.065.6221856
GGTATAC78600.065.5983355
CTTATAG79550.065.51855
CGTATAC70900.065.420695
CTATAAC68350.065.404376
TTATAGC89950.065.2925956
TATAGGG116650.065.189687
CTTATAC85700.064.8167955
GGTATAG115650.064.575855
TATAGGC106450.064.568547
TTATACG82600.064.538576
Produced by FastQC (version 0.11.5)