FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241195
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG56900.066.942456
CTATAGG88350.066.74786
GTATAGG150300.066.603436
CGTATAA83950.066.3292165
CTATAAC61700.065.9863056
TTATAGG141600.065.952926
CGTATAG87700.065.8460855
TTATAGC93950.065.784836
GCTATAC71150.065.627955
GGTATAC81600.065.581865
CTTATAG84050.065.3763965
CGTATAC64350.065.225165
GGTATAG131850.065.099395
CTATACC64350.064.953926
GCTATAG88800.064.9516455
TATAGGG146200.064.810847
GGTATAA111500.064.8093955
CCTATAC55750.064.432965
TATAGGC116400.063.704317
GTATAGA102900.063.7001086
Produced by FastQC (version 0.11.5)