FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514714
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC57670.10457477939925805No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA56700.10281584865507079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG54300.067.702826
CTATAGG78250.067.141266
GTATAGG129600.066.745656
CGTATAG78900.066.721125
CGTATAA72750.066.159065
TTATAGG122950.066.0598456
GCTATAC65550.065.955295
GGTATAG113000.065.7476045
GGTATAC71000.065.671315
TATAGGG121800.065.446917
CTTATAG69350.065.367585
CTATAAC56900.065.3468866
TTATAGC81450.065.1046456
CTTATAC65700.064.9528665
CTATACC57800.064.8740166
GGTATAA96450.064.807645
GCTATAG82600.064.706395
CCTATAC48500.064.6204765
TATAGGC103900.064.602827
GTATAGA92650.064.596426
Produced by FastQC (version 0.11.5)