FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-6435-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729373
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG54100.067.507356
CTATAGG85500.067.141386
GTATAGG129050.066.277896
CGTATAA81300.066.178585
CGTATAG78500.065.998955
GGTATAC74400.065.780395
TTATAGC84150.065.682676
CTTATAG72200.065.652875
TTATAGG125900.065.629946
GCTATAC70050.065.570465
CTATAAC61000.065.491386
TATAGGG121200.065.489577
CTATACC61950.065.2776266
GCTATAG84050.065.095455
CCTATAC51500.064.802655
GGTATAG114050.064.781395
GGTATAA96150.064.7623145
TATAGGC100350.064.281177
CGTATAC60850.064.273635
CTTATAC75700.063.911345
Produced by FastQC (version 0.11.5)