FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7486818
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC93060.12429846698557385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG109200.067.208566
CTATACG76250.066.52276
GTATAGG183650.066.477736
TTATAGG165850.066.3778156
CGTATAA105400.066.1467365
GGTATAC98050.066.0746845
CTTATAG96100.065.995775
GCTATAC90050.065.884595
CGTATAG110150.065.8349465
GGTATAG159850.065.674245
CTATAAC79800.065.097646
CCTATAC64000.065.044215
GGTATAA134500.064.891825
TATAGGG169450.064.511717
CTATACC78550.064.4857946
TTATACG102950.064.424896
TATACGG115500.064.146667
TATAGCG120200.064.024817
AGTATAG133600.063.9412165
GCTATAG113150.063.8421065
Produced by FastQC (version 0.11.5)