FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6719898
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG148300.066.5671166
CTATAGG88600.066.4104166
CGTATAA92750.066.1168065
CTATACG65450.065.848366
TTATAGG143350.065.595716
GCTATAC81900.065.393595
GGTATAG135300.065.335825
CCTATAC60950.065.3149265
CTATAAC69850.065.206146
CGTATAG91450.064.9528055
CTTATAG84400.064.658575
CTATACC73600.064.640546
GGTATAA117700.064.495175
GGTATAC86650.064.4329765
AGTATAG113050.064.363065
TATAGGG140400.064.281147
CGTATAC73550.064.208875
TATACGG92250.063.856737
GTATAGA99400.063.2068066
GATATAG104700.063.0810935