FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122164
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC50270.12195050948967581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG55600.067.374086
TTATAGG89900.067.0742346
GTATAGG100050.067.016726
CTATACG41100.066.208836
CGTATAG61550.066.202755
CTTATAG53550.066.0996555
GCTATAC51950.065.9809655
AGTATAG74950.065.846635
TATAGGG91150.065.6939247
GGTATAG85750.065.629655
CTATAAC46100.065.628636
CCTATAC37950.065.5293665
GGTATAC54000.065.419285
CGTATAA59050.065.273945
CGTATAC46100.065.2492755
GTATAGA64050.065.147766
CTATACC44450.065.074656
TATAGCG65700.065.002257
TATAGGC77300.064.840277
CTTATAC53100.064.74965
Produced by FastQC (version 0.11.5)