FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6182233
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC109310.17681313531858148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG90000.066.689256
GTATAGG155350.066.508976
GCTATAC71800.066.446335
CTTATAG77100.066.279145
TTATAGG144000.065.994576
CGTATAG90300.065.5768055
CTATACG60100.065.414326
CGTATAA81850.065.2531365
GGTATAG139400.065.111185
GGTATAC81900.064.9570545
CCTATAC52250.064.732335
CTATAAC62700.064.598446
AGTATAG121050.064.521575
TATAGGG145050.064.454797
GGTATAA106250.064.028265
CTATACC65250.063.574836
TATAGTC113950.063.568187
TATAGGC118200.063.5610167
GATATAG103600.063.5390785
TTATACG82100.063.3929256
Produced by FastQC (version 0.11.5)