FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6310068
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC202960.3216447112772794No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA97880.15511718732666588No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA63410.10049020073951659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG166650.067.2911456
CTTATAG89550.066.9491355
CTATAGG102950.066.898926
CCTATAC50450.066.836795
CGTATAA89150.066.77875
CGTATAG106000.066.656925
GGTATAG139050.066.3595665
CTATACG66750.066.288626
CTATAAC65850.066.238486
TTATAGG154000.066.209086
CTATACC66000.066.193956
GGTATAA114350.065.704915
TATAGGG175300.065.586447
GCTATAC75550.065.3269355
CGTATAC71350.065.299525
CTTATAC74000.065.277225
GGTATAC86850.065.16415
AGTATAG134550.065.14745
TATACGG113750.064.9747247
TATAACG97750.064.9107747
Produced by FastQC (version 0.11.5)