FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3357-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4568774
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC106900.2339796190400313No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA51320.11232772730715068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG73050.066.9385156
CTTATAG57150.066.6500855
GTATAGG117000.066.636676
CGTATAG69900.066.352225
CTATACG46900.066.151526
GGTATAC60300.066.126665
TTATAGG106300.065.97366
CGTATAA62000.065.893145
GGTATAG100850.065.8624955
GCTATAC51250.065.5188755
CTATACC47900.065.354666
CCTATAC40150.065.250735
AGTATAG88050.064.949755
CTATAAC50950.064.87496
TATAGGG113500.064.8379757
GTATAGA84400.064.526076
TTATAAC55650.064.424016
GGTATAA80200.064.4162145
TATACGG75650.064.312857
GATATAG79950.064.31145
Produced by FastQC (version 0.11.5)