FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6076865
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG142450.066.3230066
CTATAGG81750.066.106296
CGTATAG82350.065.7095955
CTATACG56750.065.272796
TATAGGG135650.064.9525457
GCTATAC70350.064.935355
GGTATAG132550.064.9168555
CGTATAA80000.064.8852545
TTATAGG130100.064.553086
GGTATAC76600.064.477475
CTTATAG76450.064.100695
CCTATAC53050.063.891125
GGTATAA111100.063.8492775
TTATAGC89950.063.7741666
CTATACC55950.063.6428766
GTATAGC94000.063.5940256
CTATAAC61400.063.462756
CGTATAC64200.063.4190945
AGTATAG114050.062.903165
TATACGG92550.062.8119667
Produced by FastQC (version 0.11.5)