FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6485406
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG88650.066.367976
CTATACG62000.066.347866
GTATAGG146300.066.252696
GCTATAC78050.066.05965
TTATAGG140950.065.91356
GGTATAC85950.065.644735
CGTATAA90000.065.644525
CGTATAG91050.065.425355
CTATAAC68800.065.230326
CTTATAG82050.065.226525
GGTATAA111000.065.0111165
TTATAGC96950.064.979636
CGTATAC72100.064.816055
GGTATAG138300.064.748415
TATAGGG142550.064.5587167
AGTATAG113850.064.213245
CTATACC65250.064.168966
ATTATAG120450.063.8601235
TTATAGT134100.063.6983536
TATAGCG105850.063.5792667
Produced by FastQC (version 0.11.5)