FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6227408
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG89550.067.536486
GTATAGG130850.066.563286
CTATACG60800.066.5063866
TTATAGC98650.066.377036
CTTATAG81450.066.3508455
GGTATAC78800.066.318315
TTATAGG130550.066.260746
GCTATAC81400.066.219725
CGTATAA88650.065.854685
CGTATAC67350.065.699495
CTATAAC70500.065.443246
CGTATAG86750.065.401915
TATAGGG125150.065.1783457
CTTATAC84750.065.088025
GCTATAG92100.065.0209355
AGTATAG102000.064.985755
GTTATAG122900.064.9775165
CTATACC69350.064.964866
TTATACC82450.064.952166
TATAGGC111400.064.902427