FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5544322
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC66480.11990645564958168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG53950.066.842816
GGTATAC71750.066.7373665
GTATAGG129100.066.7307056
CTATAGG80800.066.708146
GCTATAC64900.066.18225
CGTATAG80500.066.044285
CGTATAA72000.066.0198445
GGTATAG113300.065.848895
TTATAGC83300.065.629866
GGTATAA94250.065.464335
CTTATAG74700.065.412895
CTTATAC68950.065.338495
TTATAGG128100.065.258386
CGTATAC61000.065.252925
CTATAAC59400.065.067386
TATAGGG125300.064.8166967
GCTATAG84400.064.567285
GTATAGA86950.064.363246
CTATACC58000.064.286056
CCTATAA58250.064.130245
Produced by FastQC (version 0.11.5)