FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173238
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG70950.066.750735
GTATAGG117450.066.649496
TTATAGG117950.066.33736
CTATAGG74550.066.1078646
TTATAGC83050.066.080476
CTATACG51150.066.0573656
CGTATAA68800.065.989685
GCTATAC62700.065.882815
GGTATAC67500.065.706035
CGTATAG69000.065.595645
GGTATAG105900.065.5623255
TATAGGG112600.065.1079947
CTTATAC69350.064.608925
GCTATAG78250.064.501795
GGTATAA93350.064.372235
CGTATAC56250.064.358755
CTATACC54900.064.348566
CTATAAC54000.064.3198856
TATACGG78950.063.8400547
ATTATAG97400.063.670695
Produced by FastQC (version 0.11.5)