FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-3191-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4722397
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC70730.14977563301010058No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA53430.11314169477915559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG115550.067.391016
CTATAGG69450.067.093236
CGTATAA63450.066.7665945
CGTATAG72000.066.757645
CTATACG47400.066.717056
CTTATAG67300.066.637655
TTATAGC74650.066.4497156
CGTATAC53250.066.4168555
GGTATAA81050.066.295635
GGTATAC60900.066.230615
TTATAGG109650.066.135926
GCTATAC57750.066.026955
GGTATAG92750.065.966385
CTATAAC53700.065.7299966
CTTATAC63800.065.6875155
GCTATAG70550.065.551285
TATAGGG107300.065.45837
TTATACC62650.065.3856666
TATAGCG87950.065.303747
CTATACC53200.065.295666
Produced by FastQC (version 0.11.5)