FastQCFastQC Report
Wed 16 Jun 2021
HWGTLAFX2_n01_WT004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGTLAFX2_n01_WT004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37247528
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG620720.16664730072825235No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA426880.11460626326665223No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT412020.11061673676706814No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC383280.10290078847648627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)