FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287188
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT22688579.00225636168642No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT98163.4179701101717344No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG37731.3137735559981614No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT35451.2343830522166663No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT31791.1069404014095297No Hit
TAAGTAGAGTCTTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT25240.8788668050197084No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG24930.8680724821371366No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGAT23330.8123598479045085No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA20550.7155591459253172No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC19770.6883992367369111No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG12160.42341602016797364No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC8600.29945540900037604No Hit
TAAGTAGTGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT8050.28030419098291015No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT7810.27194729584801597No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA7550.26289399278521386No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC7400.25767093332590496No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTGGT7090.24687661044333328No Hit
TAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC5850.20369931891304652No Hit
TAAGTAGAGTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGGTC5570.1939496079223366No Hit
TAAGTAGAGTCTTGTGAAAGGACGAAACACCGAACCCCTACCAACTGGTC4720.1643522709862529No Hit
TAAGTAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC3680.12813905873504464No Hit
TAAGTGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC3170.11038065657339442No Hit
TAAGTAGAGTCTTGTGGAAAGGACGTAACACCGAACCCCTACCAACTGGT3140.10933604468153266No Hit
TAAGTAGAGTCTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC3100.10794322882571696No Hit
TAAGTAGAGTTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC2980.10376478125826985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)