FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36276331
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG627233817.290442079161757No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT530328014.619119006274367No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT497360813.710339118914755No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG378830510.442911109174739No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC30823898.496970104280944No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA30694798.461382161277555No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG21673435.974537502152574No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC12281123.3854360850329654No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA11680363.2198294805502794No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG3333580.9189407826276588No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT2405750.6631734615057956No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2011420.5544717297898732No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA1923620.5302686206055403No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC1360540.3750489540962674No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT868550.2394260874949013No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG850770.23452482005415598No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGG734080.20235784043320149No Hit
TAAGTAGAGTCTTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT661140.18225106612904154No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT584220.1610471577183481No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA401590.1107030366439208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)