FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69235958
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1225654217.702567212256962No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT994310314.361183534139876No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT904993713.071151553936755No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG710949810.268505275827916No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA57751008.341185948492257No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC56600618.175030957179793No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG41400095.979564838259333No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC25555793.6911152439025976No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA24118913.4835814649954No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG5255310.7590434438705969No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT4497080.6495295407048459No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3887880.5615405798241428No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA3684820.5322118890880372No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC2499810.3610566058752303No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2370070.342317788106579No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCA2215390.3199767958724569No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1751440.2529668181958282No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1532120.22128963680982072No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1422680.20548282151306407No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1346680.19450586644587195No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGG1261700.18223189747731952No Hit
TAAGTAGAGTCTTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1228010.1773659288429287No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA881390.12730234771937438No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT847090.1223482745772074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)