FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72169414
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1254705517.385557543809348No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1029021814.258419778772211No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT973783213.493017970188866No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG796162611.031856237602263No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA60106388.328511576940336No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC58637078.124919789427693No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG37609485.211276899102992No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC27596413.8238373391808334No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA25074183.474349951074842No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG5452870.755565231553633No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT4396050.6091292358283524No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3814230.5285105959153278No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA3552030.4921794155069626No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC2406820.33349584908642876No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCA2057280.28506258897986897No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC2039410.2825864707727847No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1961970.27185616333257184No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1726880.2392814219054072No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1409310.19527801625214802No Hit
TAAGTAGAGTCTTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1247000.17278787936396436No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1238540.1716156376162345No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGG1204460.16689341554027307No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT892440.12365903372860973No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA823950.11416886383475416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)