FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62030381
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1096363217.67461657216002No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT893567314.405316968793084No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT826151313.318494690529144No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG620925610.010023959066123No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA51863698.361014258480855No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC49780338.025153029448585No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG38587066.220671125653734No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC24772083.9935398752427456No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA21893143.529422139128889No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4768690.768766840235916No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT3715270.5989436047474866No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3270230.5271981160328517No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA3115770.502297414552395No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC2075740.33463279872487No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1822850.2938640663838579No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1751350.2823374565440764No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1450490.23383541687419265No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCA1351510.21787871978410064No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1339570.21595385654652033No Hit
TAAGTAGAGTCTTTTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1087880.17537857779722488No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGG1011400.16304913555181935No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1010050.16283150026113816No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTGGT735480.11856770636311262No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA733730.11828558654185922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)