FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51363091
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCA591470611.515479082051352No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC41765938.131506337887648No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC19418273.780588282741784No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT17062933.322021643907685No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG16125423.139495635104982No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG12238792.3827985741745956No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA9042641.7605326751071115No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA8855031.7240064465746423No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG5739281.1173938110539336No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2845870.5540690687793692No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2596540.5055264294744255No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCA2435770.4742257431508552No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG2045620.3982665295591342No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1473850.2869472945076456No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1438190.28000456592458584No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1238440.24111477247348687No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1145440.22300838553505278No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG1041960.20286162295022314No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGGCCCACCCAGCTCAC994190.19356117021851352No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC912190.17759639893946413No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAGGTGTAGCCTGGGAA862630.16794744693227281No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTCACGCATTGGGTGG856300.1667150444664633No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGTAACACCGAACCCCTACC773060.15050885469490144No Hit
ATACTGAATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG752410.1464884580252384No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTTTGCAGAGACTGTGC742100.14448118007539695No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC693700.13505807117410437No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGGGGAGGAGGGGCGCT671090.13065607753240552No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG608340.1184391336572793No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCTGTTCCTGTTTCAAC602870.11737416659756711No Hit
GATAGGACGCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG597950.11641628032082416No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA581840.11327978684148896No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCTTGGTTCATCTTCAG517560.10076496369737561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)