FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57326726
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA741923112.942010677532851No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC54228189.45949364001705No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC24982464.357908037518138No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT20653683.6028012484089884No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG20167513.5179943818874286No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA14824442.585956155947228No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG14520572.5329494658390224No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG7808551.3621133709955806No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA7719761.346624958139071No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA5897461.028745301100921No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT3029850.528523118518926No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA2810890.4903280190813618No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1938080.3380761706154299No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1712150.2986652333852102No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1532840.26738662870787355No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1402070.2445752789022No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1313560.2291357088838459No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA1038020.18107086736472616No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC929150.16207972525763986No Hit
ATACTGAATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG869150.15161340279575708No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGTAACACCGTACCCCTACC838090.14619533653465575No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT805150.14044932550308212No Hit
CCGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC750770.13096334857846234No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT719790.12555923741397687No Hit
GATAGGACGCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG680110.11863750949251838No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG665500.11608895997304992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)