FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22498582
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAGCTGACTTTCTGA252296011.213862278076014No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTGCTACTCTCAGCT1308980.581805555567902No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAGCTGACTTTCTGA1283000.5702581611587788No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC602840.26794577542709136No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT510750.2270143069461No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG485870.21595583223867174No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT424470.18866522343497025No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG344790.1532496581340104No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAGCTGACTTTCTGA343440.15264962031829385No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA258950.11509614250355867No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC247150.10985136752173982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)