FastQCFastQC Report
Mon 1 Feb 2021
HWGLYDRXX_n01_Cas13_del_R03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGLYDRXX_n01_Cas13_del_R03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16794588
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAGCTGACTTTCTGAC10392816.188189909749498No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAGCTGACTTTCTGAC6275993.7369121528911573No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAGCTGACTTTCTGAC350270.20856123413089978No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGGAGACGGACGTCTCT331940.19764700390387668No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG260690.15522262290685548No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG258170.15372213953685557No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT212010.12623709494987315No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT201280.11984813202919893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)