FastQCFastQC Report
Wed 27 Jan 2021
HWGC5DRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWGC5DRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859737499
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGACCT802998499.34004264015475No Hit
TTCCCGAC725305298.436357502652097No Hit
CAGTACTG716778548.33717897420687No Hit
GCAGTAGA696735118.104044674222125No Hit
GAGATGAC681631617.92836895904665No Hit
AGTAGTCT670293117.796485680567017No Hit
TGCCGTGG650079577.561372753382716No Hit
ACTTCATA615539537.159621753337062No Hit
CCCACAGT598405746.960330806740814No Hit
GGATGCCG560541836.5199183547535355No Hit
TATGATTC521041706.060474279719652No Hit
ATGCTGAA497768595.789774094755404No Hit
GGGGGGGG466593955.4271676010726155No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)