FastQCFastQC Report
Sat 25 Jan 2020
HWFWNBGXC_n02_Salt_NSF_Plate_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFWNBGXC_n02_Salt_NSF_Plate_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357770430
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38458401.0749463000617463No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT11237650.3141022582553846No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG7005600.195812717110243No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC6712660.18762478497733867No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG5282500.14765054786668647No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT4476240.12511486765409874No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC3782840.10573372427676597No Hit
TTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGA3640230.10174764862484582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC3411700.019.2527073
GGACCAT2073350.019.0872146
GGGGGTA3366250.018.71574221
GTAGATC1687000.018.68928743
ATACCGG3521100.018.6675226
GGTATCA774000.018.6429251
TCAGGGG3395400.018.50854318
TAGATCT1677400.018.4780744
GGGGTAT3419000.018.27502322
GGCTTCA3462300.018.15503314
GGTATTC3462800.017.92839824
CAGGGGG3512550.017.90096719
AGGGGGT3527950.017.85724420
GGGTATT3508000.017.84582723
TACCGGT3713600.017.7943557
TGTTATA3791350.017.7513352
TATACCG3808700.017.6794645
GTATCAA2749450.017.518211
TGGACCA2438400.017.3916655
TGGCATA3434650.017.3648736
Produced by FastQC (version 0.11.5)