FastQCFastQC Report
Sat 25 Jan 2020
HWFWNBGXC_n01_Salt_NSF_Plate_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFWNBGXC_n01_Salt_NSF_Plate_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357770430
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG57440461.6055116684741106No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC8346650.013.9564641
CCACACT8399350.013.9371592
CTCCTAC5941400.013.9036694
TCTCCTA5959100.013.8831923
CCTACGT5938600.013.8761366
CACTTGC8435700.013.8754675
AGCAGAC19032150.013.8646562
TCAGAAC4686600.013.8635942
CCTCTCT2936200.013.8614194
GTCTCCT5973650.013.8609652
TCCTACG5954050.013.8579165
TCCTTAC4294250.013.850054
AACTACC4690650.013.8480686
ACACTTG8431000.013.8442664
CTTCAGC5100750.013.8417811
TCAACCG3281700.013.8384651
CACACTT8443950.013.8381153
AGTCTCC5976200.013.8342211
CAGACTG19028600.013.8289294
AATCCTT4300150.013.8273692
Produced by FastQC (version 0.11.5)