FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28350140
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4409151.5552480516851062TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA521300.062.81355741
CTCGTAT522350.062.7466342
CGTATGC526150.062.47019244
TCGTATG526300.062.38649743
ATGCCGT527200.062.26847
TATGCCG531200.061.8312746
TGCCGTC531500.061.7896648
CCGTCTT533250.061.5474150
GCCGTCT533950.061.4545649
ATCTCGT533200.061.40477840
CGGCTAC532150.061.3049433
CATCTCG533700.061.25695439
ACGGCTA537500.061.2201632
CGTCTTC537900.061.03329551
TCACGGC548000.060.127330
GTCACGG556550.059.3576329
CACGGCT556050.059.24546431
AGTCACG562650.058.88434228
CGTCTGA578250.058.18036716
GTATGCC565800.058.1414145
Produced by FastQC (version 0.11.2)