FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37434628
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7665212.0476255300306443TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG899100.063.85381343
TCTCGTA898550.063.82070541
CTCGTAT898700.063.8024642
CGTATGC909900.063.67693744
ATGCCGT909200.063.52947247
TATGCCG913650.063.49917646
TATCTCG909250.063.49451439
TGCCGTC913600.063.22677648
GCCGTCT910850.062.9024849
CCGTCTT915000.062.73358550
ATCTCGT919000.062.67078440
CGTCTTC918200.062.2991751
GTATGCC962250.060.43906845
GCTTATC974950.060.12608736
CGTCTGA1023550.058.7067216
ACTAGCT1022450.058.06725732
TAGCTTA1020250.057.81092534
ACGTCTG1040350.057.8022615
CACTAGC1031850.057.6580331
GTCACTA1039900.057.3312429
Produced by FastQC (version 0.11.2)