FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37016317
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14259803.8523011351993772TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1648500.062.93902242
TCTCGTA1647700.062.86043541
TCGTATG1662250.062.8020843
GATCTCG1647900.062.7368839
ATGCCGT1663600.062.73303647
CGTATGC1665300.062.70206544
TATGCCG1668650.062.56221846
TGCCGTC1669900.062.4876848
CGATCAG1667550.062.45133
CCGTCTT1670900.062.4064150
ATCTCGT1658400.062.3815340
GCCGTCT1672000.062.35966549
ACGATCA1681050.062.215832
CGTCTTC1679700.062.09036351
CACGATC1685400.062.0604431
GTCACGA1690450.061.92526629
TCACGAT1694050.061.75036630
AGTCACG1712150.061.13473528
GTATGCC1713450.060.9708445
CGTCTGA1778300.059.8350316
Produced by FastQC (version 0.11.2)