FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31055556
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG12811184.125245737026894TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1669000.065.28644613
CGTCTGA1663000.065.28261616
AATCTCG1547550.065.23740439
ACGTCTG1670700.064.9887715
CACGTCT1681100.064.7385614
GCACACG1694100.064.4167911
TCTCGTA1551500.064.3754441
CTCGTAT1551300.064.3459342
CGTATGC1571500.064.3202244
TCGTATG1555450.064.2888843
GATCGGA1698550.064.20881
CACACGT1698350.064.2047112
TATGCCG1575200.064.20251546
ATCGGAA1725650.064.1444852
ATGCCGT1562750.064.01681547
TGCCGTC1564550.064.0069148
ATCTCGT1571300.063.99024640
GCCGTCT1547500.063.95647449
TCGGAAG1728450.063.9321483
CCGTCTT1554600.063.89035850
Produced by FastQC (version 0.11.2)