FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31362752
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG11746583.7453919860093907TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1352750.064.6268244
TCGTATG1351250.064.5193143
TATGCCG1357650.064.4577346
CTCGTAT1348350.064.4311542
TCTCGTA1346500.064.3559741
CATCTCG1346650.064.2943939
GCCGTCT1358250.064.16698549
TGCCGTC1361700.064.1616148
CGTCTGA1398850.064.0723716
ATGCCGT1364500.064.0584847
ATCTCGT1354200.064.0254140
CCGTCTT1362450.063.99601750
ACACGTC1409250.063.8056413
ACGTCTG1406800.063.7524815
CGTCTTC1366700.063.7097551
CACGTCT1416350.063.42733814
GTATGCC1391850.062.9034545
CACACGT1434200.062.80879212
GCACACG1435350.062.80183411
CTTAGGC1414100.061.90569333
Produced by FastQC (version 0.11.2)