FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33534082
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG466518213.911762964019712TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5329800.066.418531
ATCGGAA5399650.066.3735052
TCGGAAG5389400.066.327713
CGGAAGA5397450.066.110314
ACACGTC5331100.065.9188913
GCACACG5348600.065.8031811
CACGTCT5333750.065.75236514
CGTCTGA5320850.065.7320116
CACACGT5352100.065.7059112
ACGTCTG5326800.065.68996415
ACGATCT5179250.065.3513237
CGATCTC5188000.065.34331538
CACGATC5181350.065.3294136
GATCTCG5196000.065.3210739
TCACGAT5230000.065.1142335
TCTCGTA5228500.065.0972941
CTCGTAT5245200.065.096542
ATCACGA5252700.065.0640634
CATCACG5270400.065.021133
TGCCGTC5283400.065.0060448
Produced by FastQC (version 0.11.2)