FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30296758
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG24169507.977586248667266TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2792250.065.88781
ATCGGAA2831800.065.847332
ACACGTC2796000.065.79175613
CGTCTGA2789450.065.78322616
TCGGAAG2831550.065.76023
AATCTCG2732500.065.7209139
TCTCGTA2736950.065.7141941
CTCGTAT2740250.065.70714642
CGTATGC2758850.065.6857544
TCGTATG2756900.065.6661643
ACGTCTG2795650.065.650815
TATGCCG2761250.065.6376346
ATGCCGT2762600.065.5806847
GCCGTCT2756650.065.5711649
TGCCGTC2762300.065.5609248
ATCTCGT2745600.065.50967440
CCGTCTT2759950.065.50107650
CACGTCT2807200.065.4937414
GCACACG2814650.065.4355811
CGTCTTC2761700.065.4324351
Produced by FastQC (version 0.11.2)