FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31760530
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3524701.1097736719129057TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC412150.064.08581544
TCGTATG409500.063.97752443
TATGCCG414500.063.75658446
TCTCGTA409650.063.73610741
CTCGTAT410200.063.71784242
TGCCGTC418250.063.04430448
GCCGTCT417150.062.88775649
ATCTCGT416850.062.78402740
ATGCCGT420550.062.6343747
CCGTCTT421200.062.29067650
CATCTCG420500.062.23055339
CGTCTTC425800.061.5684451
CGTCTGA441600.061.0142516
ACACGTC445700.060.6848813
ACGTCTG453000.059.59311315
CACGTCT458350.059.0027914
GTATGCC454450.058.2578645
GATCGGA461600.058.189821
GCACACG470200.057.64060211
ATCGGAA472700.057.584142
Produced by FastQC (version 0.11.2)