FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35914584
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4558871.2693645567494254TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA520550.064.0176541
CTCGTAT520150.064.0007242
TCGTATG522800.063.82141543
CGTATGC524950.063.5869444
ATGCCGT525350.063.55849547
TATCTCG523500.063.5246339
CGCCAAT523350.063.4568733
TATGCCG527850.063.28989446
TGCCGTC532350.062.74175648
GCCGTCT533500.062.52217549
ATCTCGT534600.062.3994640
ACGCCAA536300.062.1428932
CCGTCTT537850.062.0220650
GTCACGC537750.061.9972829
CGTCTTC545900.061.13129851
TCACGCC545500.061.0992930
AGTCACG552800.060.55163628
CACGCCA554250.060.16086631
CGTCTGA563550.060.07716
ACGTCTG569300.059.48324615
Produced by FastQC (version 0.11.2)