FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34072906
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3339870.9802128412528125TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG396350.064.1854443
TCTCGTA390700.063.99215341
CTCGTAT391800.063.92725442
CGTATGC403400.063.4151444
ATGCCGT403350.063.3897147
TATGCCG406050.063.078446
TGCCGTC408350.062.612948
GATCTCG398950.062.5562239
ATCTCGT402650.062.04127540
GCCGTCT413050.061.74144749
CCGTCTT415350.061.48217850
CGTCTTC422500.060.33448451
CGTCTGA453000.057.20141616
GTATGCC448450.057.1912545
ACGTCTG463200.055.99368315
ACACGTC466000.055.78299713
CACGTCT476900.054.49363314
AGTGATC473300.053.07951736
GCTTGAA499550.051.1360759
GTGATCT489650.051.03230337
Produced by FastQC (version 0.11.2)