FastQCFastQC Report
Wed 26 Jul 2017
HWFTLBGX2_n01_mb38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34790908
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1267080.3641986003929532TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC157750.058.50960544
TCGTATG159600.057.83147443
CTCGTAT159600.057.4648642
TCTCGTA160650.056.9819641
TATGCCG162300.056.91246
TGCCGTC166800.055.3147948
AATCTCG165150.055.24056639
ATCTCGT166200.055.07889640
GCCGTCT167600.055.03024749
ATGCCGT168400.054.74848647
CCGTCTT170750.054.03502350
CGTCTTC178050.051.74126451
CGTCTGA190600.049.1000216
ACACGTC196000.047.8526513
ACGTCTG196600.047.5314315
CACGTCT203850.045.97626514
GTATGCC205700.045.07205645
GCACACG225450.041.77020311
CACACGT232000.040.5906212
ATCGGAA240500.039.7012832
Produced by FastQC (version 0.11.2)