FastQCFastQC Report
Tue 25 Jul 2017
HWFTLBGX2_n01_mb37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWFTLBGX2_n01_mb37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37218782
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4827931.2971757109085407TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT559000.064.8424242
TCTCGTA559250.064.7518341
CGTATGC562400.064.714444
TCGTATG560750.064.7134643
TATCTCG564550.064.13747439
TATGCCG568050.064.11368646
CGATGTA567150.064.0504734
ACCGATG569450.063.99727232
ATGCCGT568550.063.92421347
CACCGAT570900.063.8886931
TGCCGTC568600.063.8632148
CCGATGT570350.063.69716333
GCCGTCT568350.063.59971649
ATCTCGT571350.063.4226240
CCGTCTT571250.063.288550
GTCACCG583750.062.61490229
CGTCTTC578100.062.47167251
TCACCGA586150.062.32504730
CGTCTGA593050.062.2828316
ACACGTC599800.061.80620613
Produced by FastQC (version 0.11.2)